* Lactobacillus reuteri strain DSMZ
17648 binds to H.
All bacterial strains were incubated at 37[degrees]C for 24 hours; however, Candida albicans DSMZ
1386 was incubated at 27[degrees]C for 48 hours .
Alternatively, an antibacterial activity retesting of the crude extract of Euphorbia retusa in comparison with compounds (1-4) was carried out against Gram-positive bacteria (Micrococcus luteus DSMZ
1605, Staphylococcus warneri DSMZ
20036, and Bacillus subtilis DSMZ
704) and Gram-negative bacteria (Pseudomonas agarici DSMZ
11810 and Escherichia coli DSMZ
1058) in comparison with gentamicin (Table 6).
Human NB cell lines were obtained as follows: SK-N-AS, SH-SY5Y, SH-EP, SK-N-SH, SK-NBE(2)c, and IMR-32 from the American Type Culture Collection (ATCC) and LA-N-5 from the Leibniz-Institut DSMZ
. All NB cell lines were characterized by (i) HLA class I typing by PCR-SSP sets (Genovision) according to the instructions of the manufacturer and (ii) array comparative genomic hybridization (a-CGH) and single-nucleotide polymorphism (SNP) array analyses (see below).
Genomic DNA (gDNA) with a known heterozygous K700E mutation was obtained from the HNT34 leukemia cell line (DSMZ
Positive (supplied by DSMZ
, AS-05431) and negative (healthy soybean) control were included on each micro titer plate.
Adherent human GBM cells, GMS-10 and DBTRG-05MG, were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ
hansenii DSMZ-70238 strain was obtained from the German Collection of Microorganisms and Cell cultures (DSMZ
) and used to produce the killer toxin.
The human PCa cell lines DU145 and LNCaP as well as HEK293T cells were purchased from the German collection of microorganisms and cell cultures (DSMZ
, Braunschweig, Germany).
MS5 were obtained from DSMZ
(Braunschweig, Germany), irradiated at 60 Grays, and cultured with Gen2.2 grown in complete medium (RPMI 1640 Glutamax; GibcoBRL) supplemented with 1 mM sodium pyruvate, penicillin, streptomycin, nonessential amino acids, and 10% heat-inactivated Fetal Bovine Serum (FBS).
1077, K12 strain 343/113, DSMZ
) as the Gram-negative and Bacillus subtilis (B.
Twenty-seven strains were purchased from DSMZ
(Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany).