Isolated mRNA (product from aliquot #3) was applied to standard procedures of saliva RNA detection, and was employed as a positive control to evaluate the DSTA method performance.
We further initiated a case-control salivary biomarker study to examine the feasibility of clinical applications of the DSTA method associated with long-term ambient temperature storage.
The DSTA method is an RT-qPCR-based technique for detection of salivary transcriptomes.
The Cq value of each gene in the experimental setups [SS (DSTA), SS+DNase, and isolated mRNA] was the mean of results for samples from 5 healthy study participants, each of which was assayed in triplicate (total 15 data points).
3 for the 3 SIRGs evaluated, the mean Cq valuesdetected bySS (DSTA) increased slightlyafter 10 weeks of preservation and showed no significant difference throughout the time course [ P > 0.05; Fig.
Now I would like to talk about our new entity Defence Science and Technology Agency (DSTA) which I briefly spoke about during the Second IAPS(P) Seminar in Seoul, Korea.
Simply put, DSTA is an executive agent of Ministry of Defence and empowered by the DSTA Act passed by parliament to act on the ministry's behalf.
Defence Science and Technology Agency's mission as enshrined in the DSTA Act is "To harness science and technology to meet the defence and national security needs of Singapore."
Broadly speaking, DSTA has four main roles and functions.
Let me now highlight some of the salient points of the DSTA organisation structure.