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References in periodicals archive ?
Detergent effects of sodium deoxycholate are a major feature of an injectable phosphatidylcholine formulation used for localized fat dissolution.
Year of approval Name of the antifungal agent 1958 Amphotericin B deoxycholate 1973 5-Flucytosine 1981 Ketoconazole 1990 Fluconazole 1992 Itraconazole (oral suspension in 1997; intravenous form in 1999) 1995 Amphotericin B lipid complex 1996 Amphotericin B colloidal dispersion 1997 Liposomal amphotericin B 2001 Caspofungin 2002 Voriconazole 2005 Micafungin 2006 Anidulafungin Posaconazole (delayed release of tablet in 2013; 2006 intravenous form in 2014) 9m R 2015 Isavuconazole (Isavuconazonium) (oral and intravenous) Table 2: Spectrum of activity of antifungals against fungi causing invasive disease in critical care [13, 14].
Subsequently, KCC-32 displayed potential resistance against the toxic bile salts such as oxgall (0.3%) and sodium deoxycholate (0.5%) (Fig.
Cells were washed with PBS and the lysate was collected on ice by a cell scraper with RIPA buffer (50 mM Tris, 150 mM NaCl, 0.2% sodium deoxycholate, 0.1% SDS, 1% Triton X-100, and adjusted to pH 7.2) supplemented with protease/phosphatase inhibitors and MG132; following collection, the lysates were sonicated and then centrifuged at 14000 rpm for 10 min at 4[degrees]C.
Tween 80, stearic acid (STE), and sodium deoxycholate were purchased from Sigma-Aldrich Chemical Company (St.
After initial washing, tissues were cut into 20 x 20 [mm.sup.2] in size and placed in 1% sodium deoxycholate solution under constant agitation in a horizontal orbital shaker for 48 h.
The next procedure used the downward line of cleaning buffer solutions in the order buffers I and II (500 mM NaCl, 50 mM HEPES (pH7.5), 1% Triton-X-100, 0.1% sodium deoxycholate, and 1mM EDTA (pH 7.5)), buffer III (10 mM Tris-Cl, 250 mM LiCl, 0.5% NP-40 (pH 8.0), 0.5% sodium deoxycholate, and 1 mM EDTA (pH 7.5)), and buffer IV (1 mM EDTA, 10 mM TrisHCl).
tarda: Edwardsiella tarda; FAO: Food and Agricultural Organization; FISH: Fluorescence in Situ Hybridization; g/l: Gram per liter; [H.sub.2][O.sub.2]: Hydrogen Peroxide; [H.sub.2]S: Hydrogen Sulfide; HPCE: Higher Performance Capillary Electrophoresis; LAMP: Loop-Mediated Isothermal Amplification; LFDP: Lake Fisheries Development Working Paper; ml: Milliliter; PCR: Polymerase Chain Reaction; SIM: Sulfur Indole and Motility Test Media; TSA: Tryptic Soya Agar; TSIA: Triple Sugar Iron Agar; USA: United State of America; V/V: Volume by Volume; WHO: World Health Organization; XLD: Xylose Lysine Deoxycholate
Cells were harvested and washed twice with cold PBS, and then resuspended and lysed in RIPA buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate [SDS], 10 ng/ml phenylmethylsulfonyl fluoride, 0.03% aprotinin, 1 [micro]M sodium orthovanadate) at 4[degrees]C for 30 min after treated with LPS or SC-514.
Cells were lysed in RIPA (radioimmunoprecipitation) buffer containing 150 mM NaCl, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), 50 mM Tris-HCI (pH 8.0), and protease inhibitor cocktail (Roche Applied Science, Vienna, Austria, pH 7.4).
The cells were lysed in lysis buffer that contained 25 mM Tris-HCl (pH 7.6), 150 mM NaCl, 1% Nonidet P-40, 1% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), protease inhibitor mixture, and phosphatase inhibitors (Sigma-Aldrich, Inc., USA) and then placed on ice for 10 min.
The media used for bacteriological analysis were Soybean Casein Digest medium, Lactose broth, Peptone water Eosin-Methylene-Blue agar (EMB, Merck) and MacConkey (MC) agar (Merck), Deoxycholate Citrate Agar (DCA) and Propylene Glycol Deoxycholate Agar (PGDA) and Triple Sugar Iron Agar (TSIA).