If there is no incorporation in response to the dispensed deoxynucleotide triphosphate
, the polymerase position is indicated by a vertical line.
5 [micro]mol/L of each deoxynucleotide triphosphate
(50% Cy5-labeled dCTP and dTTP; Amersham Biosciences), 2x extension buffer, 0.
2], 200 [micro] M of each deoxynucleotide triphosphate
, 1 x PCR buffer, 1.
In brief, DNA fragments of each gene were amplified in 50-[micro]L reaction mixture by using boiled bacterial suspension with 200 [micro]M of deoxynucleotide triphosphate
, 1 [micro]M of the primer pairs, Taq buffer (QIAGEN GmbH, Hilden, Germany), and 2.
2 [micro]M for each primer, 200 [micro]M for each deoxynucleotide triphosphate