Finally, diacylglycerol o-acyltransferases (DGATs
) catalyze the acylation at the sn-3 position and give rise to triacylglycerol (TG) [35, 36].
Overall adipocyte VAT and SAT DIO2 gene expression was inversely related to markers of mitochondrial function (CIDEA, ATP5A, PGC1a, and CPT1B), fatty acid oxidation (ACADM), and fatty acid synthesis (ACC2, FASN, and DGAT
Streeper et al., "DGAT
enzymes are required for triacylglycerol synthesis and lipid droplets in adipocytes," Journal of Lipid Research, vol.
Where appropriate, the following inhibitors were used: GW9662 (10 [micro]M), inhibitor of PPAR-[gamma]; GSK0660 (10[micro]M), inhibitor of PPAR-[beta]/[delta]; SS0 (25 [micro]M), inhibitor of CD36; A922500 (100 nM), inhibitor of DGAT
enzyme; and TMP-153 (100 nM), inhibitor of ACAT enzyme.
Beyond triglyceride synthesis: the dynamic functional roles of MGAT and DGAT
enzymes in energy metabolism.
It is reasonable to expect that these genes were associated with the DGAT
Most of these enzymes encountered little change in their expression levels, except for phosphatidate phosphatase (PP) and diacylglycerol acyltransferase (DGAT
Functional analysis of three type-2 DGAT
homologue genes for triacylglycerol production in the green microalga Chlamydomonas reinliardtii.
Watt et al., "Dissociation of hepatic steatosis and insulin resistance in mice overexpressing DGAT
in the liver," Cell Metabolism, vol.
Diacylglycerol acyltransferase (DGAT
) catalyzes the final step in the biosynthesis of triacylglycerol from diacylglycerol and fatty acyl-CoA.
This occurs through the enhanced esterification of fatty acids in adipose tissue by increasing the activity of diacylglycerol acyltransferase (DGAT
), the rate limiting enzyme in TG synthesis, and indirectly enhances the activity of lipoprotein lipase (LPL) in adipocytes by increasing fatty acid trapping within adipocytes .
To compare a new digital Goldmann applanation tonometer (dGAT
) that measures intraocular pressure (IOP) in 0.1 mmHg increments to a standard Goldmann applanation tonometer (sGAT).