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DIGDiscussion Group(s)
DIGDigoxigenin (DNA marker)
DIGDeputy Inspector General
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DIGDirect Injection Gasoline (vehicles)
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DIGDéclaré d'intérêt Général (French: Declaration of General Interest)
DIGDomain Internet Groper
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DIGDiffuse Ionised Gas
DIGDisney's Internet Guide
DIGDrop in Game (informal ice hockey)
DIGDigital Information Group
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DIGDoorman is God (gaming clan)
DIGDevelopment Insight Group
DIGDelta Informal Gardeners (California)
DIGDigiorgio Corporation (NYSE Symbol)
DIGDistributed Information Gathering
DIGDigital Image Generator
DIGDevelopers Interface Guide
DIGDOE Interaction Group
DIGDead in Ground
DIGDepartement Informatique de Gestion
DIGData Item Group (US DoD)
DIGDismissed as Improperly Granted (legal)
DIGDirect Inertial Guidance
DIGDepartment Inspection Group
DIGDetonator Inspection Gauge
DIGDisaster Information Gateway
DIGDetergent-Insoluble Glycolipid-Enriched Domain (cell & molecular biology)
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References in periodicals archive ?
Sense and antisense LIF and LIFR riboprobes were generated using partial cDNAs cloned into pCRII vectors by linearizing with appropriate restriction enzymes and labeling with digoxigenin (DIG)-UTP using a DIG RNA Labeling kit (Roche, Indianapolis, IN, USA).
Plates were then washed three times with TBS-Tween 0.1% (TBS-T) buffer, and 100 mL of a 0.01 mg/mL solution of 5'digoxigenin and 3'biotin-labeled ssDNA or dsDNA was added to each well.
For ISH, the digoxigenin (DIG) DNA labeling and detection kit (Roche Molecular Biochemicals) was used to label the RLO PCR product by random priming with DIG-dUTP, and the detection was performed with an anti-DIG antibody coupled to alkaline phosphatase, followed by incubation in NBT/BCIP.
The upstream internal primer UplnSJNdv (5'-GATTTCGTTCCATTCTcTtG-3') was indicative for the SJNNV genotype and labelled with the hapten digoxigenin (Dig) at the 5' end.
These clones were labeled with digoxigenin and hybridized to the chromosomes of each species (Figure 5).
Following the isolation of DNA from the sample, the scientists performed a duplex PCR with two primer sets, of which one was labelled with biotin and the other with digoxigenin or fluorescein, respectively.
In this study, specific primers and probes for stx genes, amplification and labeling of products DIG-dUTP, hybridization of streptavidin with biotinylated probes and detection with antibody against conjugated digoxigenin and peroxidase in microtiter plates were designed.
Equilibration buffer was applied directly on the kidney sections followed by incubation with the TUNEL reaction mixture containing terminal deoxynucleotidyl transferase (TdT), digoxigenin nucleotide and unlabeled nucleotide for 1 h in a humidified chamber at 37[degrees]C.
The 1601bp FAV was transcribed in vitro with T7 RNA polymerase and transcribed RNA was simultaneously labeled with digoxigenin using DIG RNA Labeling kit (Roche), following the manufacturer's recommendations.
In the next step of procedure, an aliquot of the PCR product was denatured and hybridized to a digoxigenin labeled telomeric repeat-specific detection probe.
In our example case, consider a second potential target amplified by a forward primer with an additional label such as digoxigenin paired to a biotin-labeled reverse primer.