We also observed different alleles from codon 59 (dihydrofolate reductase
), 437 (dihydropteroate synthase), and (P.
Identification and analysis of dihydrofolate reductase
alleles from Plasmodium falciparum present at low frequency in polyclonal patient samples.
A multiplex allele specific polymerase chain reaction (MAS-PCR) on the dihydrofolate reductase
gene for the detection of Cryptosporidium parvum genotypes 1 and 2.
Point mutations in the dihydrofolate reductase
and dihydropteroate synthetase genes and in vitro susceptibility to pyrimethamine and cycloguanil of Plasmodium falciparum isolates from Papua New Guinea.
Towards an understanding of the mechanism of pyrimethaminesulfadoxine resistance in Plasmodium falciparum: genotyping of dihydrofolate reductase
and dihydropteroate synthase of Kenyan parasites.
(c) A, atovaquone; CO, cotrimoxazole; C/P, clindamycin/primaquine; D, dapsone; D+T, dapsone and trimethoprim; P, pentamidine; P+A, pentamidine and atovaquone; PM/SD (pyrimethamine/sulfadoxine inhibitors of dihydrofolate reductase
(DHFR) and DHPS, respectively); T, trimetrexate (an inhibitor of DHFR).
Sequence variations in the genes encoding dihydropteroate synthase and dihydrofolate reductase
and clinical response to sulfadoxine-pyrimethamine in patients with acute uncomplicated falciparum malaria.
Croomine was previously shown to inhibit the activity of dihydrofolate reductase
(DHFR, an enzyme for antifolate drug mechanism).
Wellems, "Pyrimethamine and proguanil resistance-conferring mutations in Plasmodium falciparum dihydrofolate reductase
: polymerase chain reaction methods for surveillance in Africa," The American Journal of Tropical Medicine and Hygiene, vol.
The following will concentrate on the enzymes that can affect BH4 levels and its relationship to histamine; Methyltetrahydrafolate reductase (MTHFR), dihydropteridine reductase, dihydrofolate reductase
(DHFR), and quinoid dihydropteridine reductase (QDPR) heavily influence BH4 (tetrahydrobiopterin) synthesis and recycling.
[sup],, In addition, a SNP 829C-T near the miR-24 binding site in the 3′ UTR of dihydrofolate reductase
was closely associated with methotrexate resistance by interfering with miR-24 function, [sup] while SNPs in miRNAs lead to altered expression levels and functions of miRNA, resulting in an increased risk of illness.
Methotrexate works by blocking the action of a key enzyme, dihydrofolate reductase
(DHFR), which cancer cells require to thrive.