2], as determined by nitroblue tetrazolium (NBT) reduction into formazan (FORZ), and dihydrorhodamine
(DHR) into rhodamine-123 (R-123) staining, respectively.
Keywords: Chronic granulomatous disease, Perirectal abscess, Nitrotetrazolium, Dihydrorhodamine
Crow, "Dichlorodihydrofluorescein and dihydrorhodamine
123 are sensitive indicators of peroxynitrite in vitro: implications for intracellular measurement of reactive nitrogen and oxygen species," Nitric Oxide--Biology and Chemistry, vol.
production and dihydrorhodamine
123 (DHR) is oxidised by the neutrophil reactive oxygen species (ROS) to rhodamine 123 (RHO) releasing a fluorescent green signal which can be measured by flow cytometry.
Mitochondrial generation of ROS was determined spectrofluorometricaily by using the fluorescent dye Dihydrorhodamine
(DHR 123) (Fukushima et al.
5 [micro]g/mL dihydrorhodamine
123 (DHR123), which was converted to rhodamine fluorescent dye by oxidation.
2] oxidizes dihydrorhodamine
to rhodamine when a peroxidase or an equivalent catalyst is present.
After finishing the insult period, exposure medium was replaced by phosphate buffered saline (PBS) containing 10 [micro]M 123 dihydrorhodamine
(123 DHR) 123 (Molecular Probes Inc.
1994 Peroxynitrite-mediated oxidation of dihydrorhodamine
Stimuli-induced ROM formation of her neutrophils was tested with dihydrorhodamine
123[Phago-Burst; Orpegen; see Ref.
In AMs, the October coarse PM was the most potent stimulator for IL-6 release, whereas the July PM consistently stimulated the highest ROS production measured by dichlorofluorescein acetate and dihydrorhodamine
Radical formation was measured at 37[degrees]C by conversion of dihydrorhodamine
123 to rhodamine 123 as a fluorogenic substrate (26, 27).