production and dihydrorhodamine 123 (DHR) is oxidised by the neutrophil reactive oxygen species (ROS) to rhodamine 123 (RHO) releasing a fluorescent green signal which can be measured by flow cytometry.
A simple flow cytometry assay using dihydrorhodamine for the measurement of the neutrophil respiratory burst in whole blood: comparison with the quantitative nitroblue tetrazolium test.
Mitochondrial generation of ROS was determined spectrofluorometricaily by using the fluorescent dye Dihydrorhodamine
(DHR 123) (Fukushima et al.
Dihydrorhodamine 123: a new flow cytometric indicator for respiratory burst activity in neutrophil granulocytes.
Diagnosis of chronic granulomatous disease and of its mode of inheritance by dihydrorhodamine 123 and flow microcytofluorometry.
A fast and easy method to determine the production of reactive oxygen intermediates by human and murine phagocytes using dihydrorhodamine 123.
Abbreviations: Bt, Bryothamnion triquetrum; DHR 123, dihydrorhodamine
123; DMEM, Dulbecco's modified Eagle's medium; DPPH, 1, 1-diphenyl-2-picrylhydrazyl radical; FBS, foetal bovine serum; MeHg, methyl mercury; NOS, nitric oxide synthase.
Radical formation was measured at 37[degrees]C by conversion of dihydrorhodamine
123 to rhodamine 123 as a fluorogenic substrate (26, 27).
In AMs, the October coarse PM was the most potent stimulator for IL-6 release, whereas the July PM consistently stimulated the highest ROS production measured by dichlorofluorescein acetate and dihydrorhodamine 123 (DHR).
Dihydrorhodamine 123 (DHR) at 5 [micro]M (Molecular Probes, Eugene, OR; catalog no.
Evaluation of 2',7'-dichlorofluorescin and dihydrorhodamine 123 as fluorescent probes for intracellular H202 in cultured endothelial cells.
Dihydrofiuorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine