on cytological smears in oral pemphigus.
((c) and (d)) Direct immunofluorescence
staining for IgA and C3 shows granular mesangial staining, respectively (400x).
Our patient's skin biopsy showed leukocytoclastic vasculitis with histopathologic evidence of IgA and IgM deposition based on direct immunofluorescence
staining but renal involvement was absent.
(DIF) was consistent with a subepithelial bullous disease.
A case-control study was done using direct immunofluorescence
on PAP smears in Hormozgan, Iran during 2004-2005.
In patients with active lesions, scrapings from lesions were collected and direct immunofluorescence
was done using commercial kit of BioRad for confirming diagnosis of chickenpox.
The biopsy is generally made at the edge of the lesion, and direct immunofluorescence
staining shows a linear deposition of IgG, IgA, or C3 along the basement membrane .
The diagnosis of disseminated discoid lupus erythematosus (DLE) was confirmed by histological and direct immunofluorescence
Comparison of Chemicon SimulFluor direct fluorescent antibody staining with cell culture and shell vial direct immunoperoxidase staining for detection of herpes simplex virus and with cytospin direct immunofluorescence
staining for detection of varicella-zoster virus.
Antinuclear antibody test, Direct Immunofluorescence
study, and Herpes simplex virus types 1&2 antibody tests were negative.
Diagnosis of DH is established on the basis of a direct immunofluorescence
test (DIF) revealing granular deposits of IgA in the papillae and the presence of circulating IgA antibodies directed against the endomysium and/or tissue and epidermal transglutaminase (tTG, eTG) [2, 3].
When detailed clinical data, such as location (face, arms, or chest), history of typical DLE, positive serologies, or direct immunofluorescence
studies, are provided, the presence of only mild keratinocytic atypia in association with corroborative histologic findings (vacuolar change, follicular plugging, dermal mucin, superficial and deep infiltrate) enable the pathologist to make a definitive diagnosis of HLE.