DTNBDtnb - 5,5'-Dithio-Bis (2-Nitrobenzoic Acid)
DTNBDorsal Tegmental Noradrenergic Bundle
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Hydrolysis of acetylthiocholine iodide/butyrylthiocholine chloride was monitored by the formation of the yellow 5-thio-2-nitrobenzoate anion, as a result of the reaction of DTNB with thiocholines, catalyzed by enzymes (at 412 nm), utilizing a 96-well microplate reader (VersaMax Molecular Devices, Sunnyvale, CA, USA).
l3]C NMR, proton and carbon-13 nuclear magnetic resonances; ACTI, acetylthiocholine iodide; MTT, thiazolyl blue tetrazolium bromide; DTNB, 5,5-dithiobis [2-nitrobenzoic acid]; NaOH, sodium hydroxide; DMSO, dimethyl sulfoxide; EMEM, Eagle's Minimum Essential Medium; FBS, fetal bovine serum; C[O.
DTNB combines with glutathione to generate a yellow product with maximal absorbance at 412 nm (Cuvette Assay for GSH/GSSG, Oxford Biomedical Research, Oxford, MI).
GSH content in root and shoot of maize was analyzed spectrophotometrically with DTNB used as chromogenic agent.
Se adicionaron a cada cavidad, 25 [micro]L de la solucion de yoduro de acetilcolina (ATCI), 125 [micro]l de la solucion de DTNB (5',5-dithio-bis-(2-nitrobenzoate, Sigma), y 50 [micro]L de Tris/HCl (50mM) con albumina serica bovina.
Here the DTNB (5, 5'-dithiobis- (2- nitrobenzoic acid) react with GSH to form GSH-TNB that absorb at 412 nm.
Disodium edetate (Reidel Dehan AG sleeze Hannover) sodium chloride NaCl (Merck) sodium hydroxide (Fluka AG) chloroform (Merck) DTNB of Sigma company cadmium acetate (Sigma) Reduced form of GSH of Fluka HCl 35% (Kolchlight) distilled water double refined potassium dihydrogen phosphate (Merck) water for injection (Elexor laboratories).