For anticancer screening the highly metastatic pediatric rhabdomyosarcoma cell line Rh30, DSMZ (Braunschweig, Germany) was chosen and cultivated in Dulbecco's modified Eagle's medium
(DMEM; Invitrogen, Germany) with 10% fetal bovine serum (FCS; PAN Biotech GmbH, Germany) and 1% gentamicin (Ratiopharm GmbH, Ulm, Germany) at 37AdegC and in a humidified atmosphere with 5% CO2.
Dulbecco's Modified Eagle's Medium
(DMEM), EDTA, Fetal bovine serum (FBS) and RPMI 1640 were purchased from Biochrom, Germany and Caco-2 cells were obtained from Cell Culture Collection, Turkey.
1: Concentration of phenol red in different media formulations from different sources (in [micro]M) Source DMEM MEM DMEM/F-12 RPMI 1640 IMDM Thermo-Fisher[R] 39.85 26.57 21.57 13.28 39.85 Lonza[R] 40.76 26.57 13.55 40.76 ATCC[R] 42.33 22.85 14.11 42.33 Sigma-Aldrich[R] 44.87 31.04 24.35 14.96 45.15 DMEM, Dulbecco's Modified Eagle's Medium
; MEM, Minimum Essential Medium; DMEM/F-12, DMEM/Ham's F12; RPMI, Roswell Park Memorial Institute; IMDM, Iscove's Modified Dulbecco's Medium.
Experiments were conducted in May, 2017 at the first affiliated hospital of the Chinese PLA General Hospital, Beijing, China.High-Glucose Dulbecco's Modified Eagle's Medium
(DMEM-H) was obtained from Hyclone (South Logan, UT), Fetal Bovine Serum (FBS) was obtained from Gibco (Waltham, MA).
Abbreviations ALP: Alkaline phosphatase CPC: Calcium phosphate cement DMEM: Dulbecco's modified Eagle's medium
ICP-OES: Inductively coupled plasma optical emission spectroscopy MTT: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide PBS: Phosphate buffer saline WF: Wollastonite fibers [alpha]-TCP: [alpha]-Tricalcium phosphate.
Dulbecco's modified Eagle's medium
(DMEM), fetal bovine serum (FBS), and antibiotics (penicillin and streptomycin sulfate) were purchased from Life Technologies Inc.
A human hepatocyte cell line HepG2/C3A was obtained from the American Type Culture Collection (ATCC CRL-10741, WCB 25022009) and cultivated in Dulbecco's modified Eagle's Medium
(Dulbecco's DMEM, GIBCO Life Technologies, Eggenstein, Germany), supplemented with 10% fetal calf serum (PAA Laboratories, Colbe, Germany), 1% of glutamine solution (PAA), and 1% of antibiotics solution (Penicillin G: 10,000 IE/mL/Streptomycin: 10 mg/mL; PAA).
C2C12 myoblasts were maintained in growth medium (GM, High Glucose Dulbecco's Modified Eagle's Medium
, Gibco, Grand Island, NY, USA) containing 1 g/L glucose with with 10% (v/v) fetal bovine serum (FBS, Gibco, Grand Island, NY, USA) at 37[degrees]C with 5% C[O.sub.2].
Cells were seeded at 2.0 x [10.sup.5] cells/well in a 6-well plate containing 2 mL Dulbecco's modified Eagle's medium
plus 10% fetal bovine serum and 1% penicillin/streptomycin.
To serve as negative controls, 3 animals were inoculated intraperitoneally with Dulbecco's Modified Eagle's Medium
. Animals were monitored for signs of clinical illness, and body weight and temperature readings were obtained daily.
Reagents: F-12K, Dulbecco's Modified Eagle's Medium
(DMEM) culture media, Phosphate- buffered saline (PBS), Trypsin EDTA, Fetal bovine serum (FBS), Penicillin-Streptomycin, A549 human lung cancer cell line, and MRC-5 human normal lung cells line were each purchased from American Type Culture Collection (ATCC) located in Manassas, VA, USA.
Dulbecco's modified Eagle's medium
(DMEM), L-glutamine, Antibiotic-Antimycotic (lOOx), trypsin-EDTA, [H.sub.2][O.sub.2] and collagen were obtained from Invitrogen (Carlsbad, CA, USA).