Effect of EAPL on activities of SOD, GSH-Px and levels of MDA in the hippocampus
Treatment with EAPL 200mg/kg and 400mg/kg clearly inhibited the decreases in SOD and GSH-Px activities, and the activities of SOD and GSH-px were increased to 179.
Effect of EAPL on neuronal apoptosis in the hippocampus
Treatment with EAPL 200mg/kg and 400mg/kg markedly attenuated neuronal apoptosis, and the number of TUNEL-positive neurons was reduced to 5.
Treatment with EAPL 200mg/kg and 400mg/kg clearly reduced the expression of p-JNK from 2.
Treatment with EAPL 200 mg/kg and 400 mg/kg markedly inhibited the increases in the relative ratio of Bax/Bcl-2.
LC-MS fingerprint and identification of major peaks in EAPL
There were more than 32 major peaks in the LC-MS fingerprint chromatogram of EAPL (Fig.
It has been reported that EAPL has neuroprotective effects against ischemia/reperfusion (I/R) damage, excitotoxic injury and hypoxia-reoxygen injury due to improving the redox imbalance and inhibiting apoptosis (Sun et al.
However, EAPL markedly reduced the latency, significantly increased the time in the first quadrant and the number of the target crossings.
However, EAPL increased SOD and GSH-Px activities while reducing the MDA level.
Also, EAPL could markedly reduce the number of TUNEL-positive neurons and attenuate the ultrastructural damage to the hippocampus neurons, which suggests that EAPL is able to suppress A[beta]-induced neuronal apoptosis.