Stimulation of PR[2X.sub.7] by eATP results in rapid opening of a ligand-gated cation channel, followed by induction of a cytoplasmic pore via pannexin-1, which triggers [K.sup.+] efflux and allows danger signals to access the cytosol and activate the nucleotide-binding and oligomerization domain (NOD), leucine-rich repeat, and pyrin domain containing (NLRP) 3 inflammasome .
Hepatic levels of eATP were assessed colorimetrically in liver tissue homogenates using the ATP assay kit (ab83355, Abcam, Cambridge, UK) as per manufacturer's instructions.
Levels of eATP increased significantly in HFD-fed WT and to a lesser extent Pr[2x.sub.7.sup.-/-] mice (Figure 4(d)).
These data support the concept that the protection afforded by Pr[2x.sub.7] gene deletion was related not only to reduction of the proinflammatory and profibrotic response to the HFD but also to decreased lipotoxicity driving hepatocyte injury and release of danger signals such as eATP, which activate the innate immune system.
In fact, dead hepatocytes may have released eATP  and uric acid , thus inducing pore formation via PR[2X.sub.7] and frustrated phagocytosis with consequent lysosomal disintegration and release of proteases, respectively .
Immunohistochemistry for PR[2X.sub.7] in liver sections from representative animals ((a); original magnification 100x) and quantification of liver PR[2X.sub.7] protein content (b), Pr[2x.sub.7] mRNA expression (c), and eATP levels (d) in NFD- and HFD-fed WT and P[2x.sub.7][r.sup.-/-] mice (mean [+ or -] SD; n = 4-6 per group).
Bill to the less generous VEAP was responsible for a reduction in high-quality enlistments was bolstered by the Educational Assistance Test Program (EATP
) in the early 1980s, which provided experimental evidence that education benefits had value as an enlistment incentive (Fernandez 1982).