Meru et al (165) published a careful study in 2001 in which they demonstrated highly variable but generally elevated levels of EBER1
and EBER2 in the tonsils of pediatric recipients of liver transplants (range, 2-1000/0.5 [cm.sup.2]; mean, 434/0.5 [cm.sup.2]), as compared with controls (range, 1-200/0.5 [cm.sup.2]; mean, 47/0.5 [cm.sup.2]), but no evidence of lytic infection or latency III proteins.
may also increase the expression of IGF-1, an autocrine growth factor which accelerates cell proliferation in EBVaGC .
In situ hybridization for EBER was performed using a 30-base oligonucleotide complementary to a portion of EBER1
, a region of the EBV genome that is actively transcribed in latently infected cells.
Epstein-Barr virus was detected by in situ hybridization of EBV-encoded RNA 1 (EBER1) transcripts using complementary probes as previously described. The EBERs are the most abundant transcripts in cells with latent EBV infection.
All of the 10 smooth muscle biopsies were negative for EBER1.
An in situ hybridization study for Epstein-Barr virus-encoded (EBER1) mRNA was performed using the probe and detection system from Novocastra (Newcastle upon Tyne, United Kingdom).
Although Epstein-Barr virus has been demonstrated in selected cases of FDC sarcomas,[2,3,23-25] it is unlikely that this virus plays an important role as an etiologic agent, and our case did not reveal any nuclear signal on EBER1 mRNA in situ hybridization, which is the most specific and sensitive method for the detection of Epstein-Barr virus.
In situ hybridization studies for Epstein-Barr virus (EBV) ribonucleic acid (RNA) were performed using a 30-base oligonucleotide probe complementary to a portion of the EBER1
gene and methods previously described by others.