In those patients with evidence of thyroid dysfunction, autoimmune thyroiditis was ruled out by estimating levels of antithyroglobulin and antithyroperoxidase by ECLIA
The other antibody, covalently linked with a ruthenium complex, is used for ECLIA
Serum P4 concentrations were determined by electrochemiluminiscence immunoassay (ECLIA
) using a commercial test kit (Elecsys Progesterone II, Roche Diagnostics GmbH, Germany) in an immunologic test analyser (cobas e 601, Roche Diagnostics GmbH, Germany).
In our clinic, calcidiol is measured with the COBAS 6000 Entegre device using the ECLIA
(electrochemiluminescent immunoassay) method.
Serum levels of ucOC were measured using an electrochemiluminescence immunoassay (ECLIA
) kit (Picolumi ucOC; Sanko Junyaku, Ibaraki, Japan) ().
Serum TSH, f[T.sub.3], and f[T.sub.4] levels were determined by using a Cobos e601 analyzer (Roche HITACHI Germany) with electrochemiluminescence immunoassay (ECLIA
Serum ferrritin elektrokemiluminesans immunolojik test metodu (ECLIA
) ile, demir ve demir baglama fotometrik test metodu ile, CRP degerleri immunoturbidimetrik test metodu ile (cobas 6000; Roche, Tokyo,Japonya) otoanalizorde olculdu.
Glucose was analyzed by enzyme immunoassay (Cobas 8000, Roche Diagnostics, Basel, Switzerland), [HbA.sub.1c] by turbidimetric inhibition immunoassay (Roche Diagnostics), insulin by DELFIA method (Perkin Elmer, Waltham, Massachusetts, USA), and C-peptide by electrochemiluminescence immunoassay (ECLIA
) (Roche Diagnostics).
Cortisol was measured with ElektroChemiLumineszenz ImmunoAssay "ECLIA
" Elecsys and cobas e Immunoassay System with a lower detection limit of 0.500 nmol/L.
Plasma intact PTH was measured by an electrochemiluminescence immunoassay (ECLIA
) run on a Cobas 6000 autoanalyzer (Roche Diagnostics, Mannheim, Germany; reference range 10-65 pg/mL).