Samples were ground and polished using a Buehler Ecomet
3 Variable Speed Grinder/Polisher interfaced with an Automet 2 Power Head (2).
Her father, Ecomet
Burley Sr., was an elementary school principal, while her mother, W.M.
The sample surfaces were planed with a water-cooled automatic grinding/polishing machine with 600, 1,000, 1,200 and 4,000 grit sandpaper discs under running water for 30 s (#600) and 60 s and polished with diamond paste (3 and 1 [micro]m; Ecomet
3, Bueller, USA).
Sections were embedded in epoxy resin (Resigel--Redefibra de Sao Paulo) and polished in a rotary polisher (Ecomet
4, Buehler, Lake Bluff, USA), with 600, 1000, 1200, 2000 and 4000 grit abrasive paper, and final polishing was completed with a felt disk and diamond paste of 1im and 1/4im (Arotec, Sao Paulo, Brazil).
Blocks were then sectioned with the low-speed saw and polished to the desired level with a Buehler Ecomet
Enamel surfaces were flattened with 120-, 240-, 400-, and 600-grit silicon carbide papers on a polisher machine (Ecomet
3, Buehler Ltd., Lake Bluff, IL, USA), in order to expose the surface dentin, and standardize the smear layer.