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According to the EDSTP, the compounds will be tested for effects on the estrogen, androgen, and thyroid systems in humans, fish, and wildlife by a combination of in vitro and in vivo screening assays.
Short-term estrogenicity assays in the EDSTP. The validity and usefulness of some of the assays included in the EDSTP and other short-term estrogenicity assays was recently evaluated in a comparison study by Andersen et al.
There are several attractive short-term assays that are not included in the EDSTP, including the cell proliferation assay (E-SCREEN) described by Soto et al.