After defining the intra-osseous microcirculation level, without changing the probe position, were carried out on the samples the endothelium dependent vasodilation (EDVD
) in response to a single intravenous injection of a solution of acetylcholine dose of 40 mg/kg  and endothelial nondependent vasodilation (ENVD) in response to a single intravenous injection of sodium nitroprusside solution at a dose of 30 mg/kg .
To determine the degree of manifestation of endothelial dysfunction we've studied index of the endothelium-dependent vasodilation (EDVD) of brachial artery.
Amongst 82 % patients of the 2nd group EDVD was only 5,4 [+ or -] 0,15%, what is significantly less, than in 1-st and control groups (p<0,05).
A high-resolution external ultrasound instrument (Acuson 128XP/10) with a 7.5-MHz linear array transducer (Toshiba SSH-140A) was used to measure changes in brachial artery diameter in response to reactive hyperemia [endothelium-dependent vasodilatation (EDVD)], as described by Celermajer et al.
Because the agreement of EDVD measurements among our trained personnel was high, a single observer who was blinded to the participants' clinical and biochemical characteristics analyzed the scans recorded in the present study.
We observed no association between circulating lactoferrin concentration and EDVD(r = 0.03; P = 0.7); however, in the subset of obese participants with an altered glucose tolerance (n = 15), we found circulating lactoferrin concentration to be positively associated with EDVD (r = 0.54; P = 0.04).
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