EGRLEquatorial Geophysical Research Laboratory (Mumbai, India)
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In the case of relatively highly expressed genes such as Actb, we were able to isolate and amplify mRNA at all 4 time points; however, for genes with lower expression (e.g., Egrl), we did not detect amplified product after 24 h, but only after [greater than or equal to]48 h of culture (results not shown).
The research proposal went through a rigorous review process, involving scrutiny by outside scientists as well as consideration by the Levy Board's veterinary advisory committee, before the board made its decision to direct the EGRL money to the research.