During September 2016-February 2017, we routinely tested 265 field samples by using specific EHDV real-time reverse transcription PCR (rRT-PCR), as described previously (6).
However, the deaths of EHDV-positive cattle probably were not caused by EHDV. In 1 such case, in an adult dairy cow, Escherichia coli from all tested internal organs was isolated, and Babesia spp.
The NovareDx EHDV
Strip Test uses a monoclonal antibody against EHDV
surface protein, increasing the sensitivity and specificity of the assay.
El VLA y EHDV
se trasmiten a rumiantes domesticos y silvestres y otros mamiferos a traves de insectos del genero Culicoides.
All samples were positive for EHDV
genomes by reverse transcription PCR (RT-PCR) (6).
The NovareDx - EHDV
Detection Kit is a sandwich ELISA using a purified EHDV-specific monoclonal antibody as the capture antibody and a second monoclonal antibody that binds to a different EHDV
epitope as the detection antibody.
serotype 2 (EHDV-2) strain Ibaraki virus was first identified in affected cattle in Japan in 1959.
To confirm the EHDV
serogroup, we performed reverse transcription PCR.
The aim of this study was to identify 3 particular orbiviruses (BTV, EHDV
, and AHSV) in Algeria and determine their geographic distribution.
To distinguish between BTV and EHDV
, samples positive by indirect ELISA were tested for BTV antibodies with the competitive ELISA produced by the Institute for Animal Health (Pirbright, UK).
act as vectors, transmitting EHDV
between susceptible ruminant hosts (2).
Despite the different placement of the 7 TOV genome segments, they were all more closely related to BTV than to any of the other known orbivirus species, in particular to EHDV
, which is the closest relative to BTV (Figures 2, 3).