A commercial ELISA test kit has been developed using EHV-1/4 recombinant glycoprotein G for the detection and distinguishing EHV-1 and EHV-4 infections such as SvanovirA(r) and has been used broadly in to detect EHV-1 and EHV-4 infections (Ataseven et al., 2009; Brown et al., 2007; Dynon et al., 2007; Gur and Yapici, 2008).
CF, along with other serological assays is suitable for ascertaining antibodies against EHV-1 or EHV-4. CF antibodies have low vitality which is normally untraceable for three months when infection happen (Thomson et al., 1976).
Direct immunofluorescence detection of EHV-1 or EHV-4 antigens in cryostat sections of tissues freshly dissected from aborted foetuses offers a rapid technique for the diagnostic laboratory to conduct an early diagnosis of EHV abortion (Allen et al., 2004).
Furthermore, it also can be attempted on infected cell monolayers for both EHV-1 and EHV-4 detection (van Maanen et al., 2000).
The latency site for EHV-1 and EHV-4 have been found in the lymphoid tissue debilitating the respiratory tract (Chesters et al., 1997; Edington et al., 1994; Slater et al., 1994) and in the peripheral blood (Chesters et al., 1997; Smith et al., 1998; Welch et al., 1992).
Conventional Polymerase Chain Reaction: PCR in detection of EHV-1 and EHV-4 is considered superior to virus isolation in terms of rapidness, sensitivity and specificity (Diallo et al., 2007; Marenzoni et al., 2008; Varrasso et al., 2001).