ELLSAEnzyme Linked Lectin Specific Assay
ELLSAEnzyme Linked Ligand Sorbent Assay
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2006), the effect of Pectinex treatment in species from the Nepetoideae subfamily is notorious, thereby allowing the ELLSA assay to be used for detecting high lectin activity in species which otherwise would have been classified as lacking: it wich is the case of Melissa officinalis, Origanum vulgare, Salvia amethystyna subp amethystyna, S.
Comparing these results with those obtained by ELLSA assay (Tables 3 and 4) it was observed that the presence of Tn antigenrecognising lectins could be detected in all species.
This discrepancy is explainable if one considers that pectins interfering with detecting lectins were not eliminated in such work and that erythroagglutination is notoriously less sensitive than the ELLSA assay.
Using the ELLSA assay for detecting lectins instead of erythroagglutination offers greater sensitivity since it allows lower amounts of lectin to be observed at the same time as supplying semi-quantitative data about a protein's relative abundance.
However, the company plans to shift its focus on developing its ADAPT system therapies, and thus plans to license or sell ELLSA to another company that specialises in research diagnostics.
The introductory price for each ELLSA order placed before 30 November 2011 is USD179 (EUR129) per kit.
The criteria used for evaluating whether there was recognition of the Tn antigen was based on the ELLSA assay given that it is more sensitive than erythroagglutination, meaning that negative results obtained with the latter do not necessarily mean the absence of lectins.
However, activity was observed using ELLSA (33-64%) which appreciably increased with Pectinex digestion (Table 2).
The results obtained by ELLSA established the presence of Tn-specific lectins in the Hyptis genus since values indicating high lectin activity were even obtained in extracts which had not been treated with Pectinex, except H.
The ELLSA assay revealed that high Tn-specific lectin activity could be detected in all species (Table 3) and that treatment with Pectinex frequently increased observed activity, just like that observed in previous work (Fernandez-Alonso et al.
Comparing these results with those obtained in ELLSA assay (Table 3), it can be seen that the presence of lectins recognising the Tn antigen could be detected in all the species, including those producing negative results for erythroagglutination.