One of the most spectacular contributions made possible by EMCCD
cameras was the development of super-resolution localization microscopy.
Images were captured on an electron multiplying charge coupled device camera (EMCCD
Evolve 512, Roper) with 512 x 512 pixels.
An acquisition time of 300 ms and an EMCCD
gain of 3800 were used for both systems, for all samples, except for the GFP control.
Ionita et al., "Image acquisition, geometric correction and display of images from a 2x2 x-ray detector array based on electron multiplying charge coupled device (EMCCD
) technology," in Medical Imaging 2013: Physics of Medical Imaging, vol.
An intensified EMCCD
camera for low energy gamma ray imaging applications.
Unlike Electron Multiplying CCD cameras (EMCCD
), the PIC-75 does not require cooling of its CCD sensor, thus avoiding condensation problems.
To image the light emitted from the 605 nm QD we used a custom made filter cube (Leica HQ420/40x + 1064, z440/1065rpc, and HQ605/40m-2p) which allowed the 420 nm light from the mercury lamp to be reflected up to the sample and only allowed the emitted 605 nm light to be transmitted to the EMCCD
camera (Ixon, Andor).
Fluorescence intensity maps were measured using a Nikon Eclipse Ti inverted wide-field microscope equipped with an Andor iXon Du-888 EMCCD
detector cooled to -75[degrees]C for low dark current values.
The 18-kg turret can carry up to seven payloads, which include the new diode-pumped LD module, an EMCCD
low-light tactical laser rangefinder, an eye-safe laser rangefinder and a laser illuminator/pointer.
Its products include low light EMCCD
The solution reportedly enhances the day-to-day quantitative reliability of the technology, and circumvents the need to return back-illuminated EMCCD
cameras to the factory for recalibration.