One of the most spectacular contributions made possible by EMCCD
cameras was the development of super-resolution localization microscopy.
Investigation of the intrinsic spatial resolution of an intensified EMCCD
The images captured in WF and TIRFM by the EMCCD
camera are compared in Fig.
According to the company, the EM gain of back-illuminated EMCCDs
, by any manufacturer, will deplete over time, the rate dependent on the extent of gain applied and on typical signal intensity incident on the detector.
sensor used in this camera provides programmable, solid-state gain which amplifies the photon generated charge before it reaches the readout amplifier, thus boosting the signal above the amplifier noise.
High Performance EMCCD
& CCD Cameras for Life Sciences
Princeton Instruments designs and manufactures high-performance CCD, ICCD, and EMCCD
cameras; spectrographs; and optics-based solutions for the scientific research, industrial imaging, and OEM communities.
Tenders are invited for Supply of EMCCD
Abbreviations: BRET, bioluminescence resonance energy transfer: cAMP, cyclic adenosine monophosphate; CFP, cyan fluorescent protein: cpFP, circularly permuted fluorescent protein: Cy, cyanine: DAMP, damage-associated molecular pattern: deac, diethylaminocoumarin: EMCCD
, electron-multiplying charge-coupled device; ENT, equilibrative nucleoside transporter: FMLP.
A good example of this approach is found in Quant-View imaging, first developed for Photometrics' Evolve 512 EMCCD
When eXcelon technology is applied to popular EMCCD
devices, the result is a detector with sub-electron read noise, sensitivity, low dark current, minimal if any etaloning, and high frame rates.
Photometrics, a leading designer and manufacturer of high-performance CCD and EMCCD
cameras for life science applications, has announced the introduction of a comprehensive Customer Assurance Plan to assist researchers in meeting research grant productivity goals.