EMCVEncephalomyocarditis Virus
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(144) These findings suggest the physiological function of Pr[P.sup.C] is related to the cellular antioxidant defense system: viz., loss of the antioxidant defense system in [Prnp.sup.-/-] mice may contribute to more severe lesions in the brain after EMCV infection.
(2008) Cellular prion protein prevents brain damage after encephalomyocarditis virus (EMCV) infection in mice.
DENV-2, EMCV, and VACV-WR were kindly donated by Dr.
The cell monolayer was infected with viral suspensions with titers of 2.5 x [10.sup.6]; 1.0 x [10.sup.6]; 1.0 x [10.sup.6]; and 1.0 x [10.sup.4] [TCID.sub.50]/mL, respectively, for HSV-1, EMCV, VACV-WR, and DENV-2 [1].
Serum samples obtained at different times postinoculation (6, 12, 24 hpi) from mice inoculated with iPPVO or controls (iBoHV-1 and iVACV) were assayed for IFN-I activity against murine EMCV according to a previous study (27).
Plates were then drained, washed 3 times with MEM, inoculated with 100 [TCID.sub.50] EMCV, and incubated for 2 h at 37[degrees]C.
Dengue virus 2 (DEN-2), Encephalomyocarditis murine virus (EMCV) and Vaccinia virus strain Western Reserve (VACV-WR) were kindly donated by Dr.
Human EMCV infection and disease have been documented by virus isolation from several specimen types, including serum, stool samples, cerebral spinal fluid, and throat washings (10-12).
In this study, we used broad-range nested reverse transcription--PCR (RT-PCR) targeted at domains conserved between the Saffold prototype virus, various TMEV strains, and EMCV. We screened 844 patients from all age groups with acute enteritis, including 39 controls, in 3 independent cohorts from 2 countries (Germany and Brazil) on 2 continents.
For this, murine encephalomyocarditis virus (EMCV)-sensitive L 929 fibroblasts were incubated with different amounts of EPs[R] 7630 and some characteristic constituents, respectively, before exposure to EMCV suspensions.
For comparison, the EF and CD loop structure sequences of EMCV and Mengovirus (2 members of the EMCV species) have 95.2% and 95.1% aa identities, respectively.
Interferon activity was assessed as capacity to protect murine L929 fibroblasts from the cytopathic effect of murine encephalomyocarditis virus (EMCV, generously provided by Prof.