I moved into writing the roles I wanted as a script writer and back to leading roles with 'Games Men Play,' which I co-wrote with Emem
Isong to investing back into the industry that changed my life.'
Martin, Karim and Emem
all mentioned a development scheme that the company runs called Career Choice.
A total of 11 wells showed CPE, and putative virus culture from these wells was passaged a second time by inoculation of a 24-well plate containing 80% confluent Vero cells in EMEM
with 80 pL of culture supernatant from each positive well.
HEp-2 cells were maintained in EMEM
(Lonza, Belgium) supplemented with 2% fetal bovine serum (FBS) (Lonza, Belgium), 100 U/ml penicillin and 100 [micro]g/ml streptomycin at 37[degrees]C in 5 % C[O.sub.2] and subcultured every 3-4 days.
An 18-gauge needle (TERMO, Japan) was introduced into the opening at the knee-joint end of each bone, and the marrow was washed out of the bone shaft by EMEM
. The obtained marrow pellet was separated by trituration, and the cell suspensions obtained from all the bones were combined by centrifugation.
Human normal BJ foreskin fibroblasts (ATCC[R] CRL-2522[TM]) and human normal MRC-5 lung fibroblasts (ATCC CCL-171[TM]) were purchased from ATCC (Middlesex, UK) and cultured in EMEM
(Gibco[R], Thermo Fisher Scientific Inc., Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific).
There were 74.50% males compared to studies by Desai et al.,  Cillino et al.,  and Emem
and Uwemedimbuk  who had 83%, 84.6%, 74%, and 61.4%, respectively.
The human nasal septum quasidiploid tumor cell line RPMI2650 was maintained in Eagle' s minimal essential medium (EMEM
) with 2 mM glutamine, 1% nonessential amino acids, 10% FBS, 100 U/mL penicillin, and 100 [micro]g/mL streptomycin.
Udofia holds a PhD degree in Library and Information Science with specialization in Information Technology.
T98G cells were cultured in Eagle's minimum essential medium (EMEM
; Euroclone SpA, MI, Italy) supplemented with 10% foetal bovine serum (Sigma-Aldrich, St.
Briefly, the U-87MG cells were grown in Eagle's Minimum Essential Medium (EMEM
, GIBCO) containing 10% heat-inactivated fetal bovine serum (FBS) and penicillin/streptomycin (pen: 100 I.U./mL; strep: 100 [micro]g/mL).
Maoto powder was dissolved and incubated in warm EMEM
for 1 hr.