8] leptospiras/ml de EMJH
es equivalente a una densidad optica de 0.
Microscopic agglutination test (MAT) is performed with a battery of 10 live cultures (Pomona, Icterohemorrhagiae, Australis, Autumnalis, Hebdomadis, Canicola, Louisiana, Grippotyphosa, Javanica and Patoc), which were grown in EMJH medium.
Growth was confirmed by intermittent observation of EMJH medium by Dark field microscopy and Dinger's ring formation just 1-2 cm below the surface of medium.
Becton Dickinson and Company (2000) Leptospira medium base EMJH
Silvio de Arruda Vasconcelos, FMVZ, USP, foi mantida a partir da inoculacao intraperitoneal em hamster e, posteriormente, em meio de cultura EMJH liquido e Fletcher para a producao de inoculo para a infeccao dos animais.
Para infeccao dos animais do Grupo 2 utilizou-se 1,0mL de EMJH, contendo 1,45 x [10.
Para a contagem e posterior inoculacao, a amostra foi mantida em EMJH.
interrogans the medium employed was Ellinghausen-McCullough-Johnson-Harris (EMJH
) supplemented with 10% EMJH
(Difco, USA), and incubation at 30[degrees]C for seven days.
em meio de EMJH
, foram utilizadas para o preparo do antigeno.
growth ring was observed in Fletcher medium, the culture was subcultured into EMJH without antimicrobials (FAINE et al.
From the EMJH medium with growth of Leptospira spp.
Bacterial strain and cultivation: Leptospira interrogans serovar Hardjo subtype hardjoprajitno (WHO strain) was grown in three liters of EMJH
liquid medium at 30[degrees]C (ELLINGHAUSEN and MCCULLOUGH, 1965) for at least 1 week.