ENC1Ectodermal-Neural Cortex 1
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In Helisoma trivolvis, the occurrence of this rotation is accompanied by the differentiation of a pair of embryonic serotonergic neurons identified as ENC1s (Goldberg and Kater, 1989; Diefenbach et al.
Only those with both ENC1s clearly visible were used (about 10% of stage E25 embryos).
To test the role of ENC1s in regulating embryonic rotation, stage E25 embryos with ENG1s ablated were transplanted into new egg capsules and embryonic rotation was monitored the following day with a CCD video camera (JVC, TK860U) mounted on a dissecting microscope (Zeiss, StemiSR) Rotation was recorded at 2.