To mimic a real diagnostic situation, the novel assay was used in a blinded manner in a quality assurance study of the ENIVD. The sample panel contained different orthopox viruses added to human plasma.
The good performance of our test in the international ENIVD quality-assurance study on PCR for orthopox viruses further underlines that our test is technically optimized.
To receive feedback on performance of this method and other methods of CHIKV detection, a proficiency study was organized among ENIVD members.
ENIVD attempted to assist implementation of molecular diagnostics on an ad hoc basis by distributing a validated CHIKV RT-PCR and all required reagents.
To evaluate the sensitivity of the SARS-CoV-specific assays, RT-PCRs were performed repeatedly on serial dilutions of RNA extracted with the Viral RNA Kit (Qiagen) from cultured SARS-CoV with defined amounts of genome equivalents (GE) by using the international standard of the European Network for the Diagnostics of Imported Viral Diseases (ENIVD
), distributed through the Robert Koch-Institut (available from: http://www.