ENIVDEuropean Network for Imported Viral Diseases
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To mimic a real diagnostic situation, the novel assay was used in a blinded manner in a quality assurance study of the ENIVD. The sample panel contained different orthopox viruses added to human plasma.
The good performance of our test in the international ENIVD quality-assurance study on PCR for orthopox viruses further underlines that our test is technically optimized.
To receive feedback on performance of this method and other methods of CHIKV detection, a proficiency study was organized among ENIVD members.
ENIVD attempted to assist implementation of molecular diagnostics on an ad hoc basis by distributing a validated CHIKV RT-PCR and all required reagents.
To evaluate the sensitivity of the SARS-CoV-specific assays, RT-PCRs were performed repeatedly on serial dilutions of RNA extracted with the Viral RNA Kit (Qiagen) from cultured SARS-CoV with defined amounts of genome equivalents (GE) by using the international standard of the European Network for the Diagnostics of Imported Viral Diseases (ENIVD), distributed through the Robert Koch-Institut (available from: http://www.