To evaluate the potential synergistic effect of EOMS or its essential component used in combination with ACV, all the compounds were used at sub-inhibitory concentrations.
Next, to identify whether EOMS and PEO had the ability to inhibit viral replication in other steps (before or during viral adsorption), Vero cells were pre-incubated with 30 [micro]g/ml of the compounds for 1 h at 37[degrees]C before HSV-1 adsorption.
These results strengthen the specificity of the two oils, EOMS and its main component PEO, in inhibiting HSV-1 replication.
The antiviral activity of EOMS and PEO was due to inhibition of specific steps of virus life-cycle.
In conclusion, the results we obtained in vitro are encouraging and it is strongly advisable to further explore the antiviral properties of EOMS and PEO in vivo models of viral infections.