EPGNEOS Polar Ground Network
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High pressure was then applied for 30 min to the microwells containing PSA:anti-PSA, whereas pressure was applied for 20 min to those containing EPGN: AE3.
Although the absorbance measurements from the wells containing the EPGN:AE3 complex did not portray the same trend with increasing pressure as the PSA:anti-PSA system, absorbances from pressurized wells containing EPGN:AE3 nevertheless were significantly lower than the control wells at all pressures tested.
As shown in Table 4, antibodies that had been dissociated at 140 MPa and 275 MPa were able to rebind to immobilized EPGN. In contrast, antibodies that had been dissociated at 415 MPa and 550 MPa did not rebind to the solid phase.