Two key markers of the beta arrestin pathway - ERK1
and MUC5AC - which are necessary for the activation of mucous metaplasia in the airway, showed the most robust changes.
Rhamm-/- fibroblasts are defective in CD44-mediated ERK1
,2 motogenic signaling, leading to defective skin wound repair.
and ERK2 are a critical nexus between the surge in luteinizing hormone and ovulation, said Dr Louis V.
Compared with the control, the expressions of ERK1
, ERK2, p90RSK, ZO-1, claudin-1, occludin mRNA increased 85%, 195%, 47%, 29%, 120%, and 54% respectively in the 10 nmol/L GLP-2-treated group and 301%, 747%, 883%, 59%, 251%, and 142% respectively in the 100 nmol/L GLP-2-treated group while the abundance of total ERK1
/2 protein was unchanged (p>0.
/2 phosphorylation by BPA was abolished by silencing GPER expression (Figures 1D, 2C), suggesting that GPER is required for ERK1
/2 activation after exposure to BPA.
The phosphorylation of ERK1
and ERK2 was up-regulated in response to BPA25, but not BPA2500, treatment.
In the current study, a rat pituitary cancer cell line was used to study the effect of alkylphenols and BPA on ERK1
and ERK2 activation (measured as phosphorylation), both alone and in combination with each physiologic estrogen.