Transepithelial electrical resistance TEER was measured using an EVOM
epithelial volt/ohm meter (B).
Abbreviations A apical B basolateral BBB blood-brain barrier BCEC brain capillary endothelial cells BCSFB blood cerebrospinal fluid barrier CNS central nervous system DAPI 4',6-diamidin-2-phenylindol DMSO dimethylsulfoxide EDTA ethylenediaminetetraacetic acid EVOM
epithelial voltohm meter FBS fetal bovine serum HBSS Hank's balanced salt solution MIT 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide NEAA non-essential amino acids NMDA N-methyl- d -aspartate [P.sub.app] apparent permeability coefficient [cm/s] PBS phosphate buffered saline SE hydroethanolic saffron extract SGF simulated gastric fluids SIF simulated intestinal fluids TFA trifluoroacetic acid TEER transepithelial electrical resistance Acknowledgment
The measuring instrumentation consisted of an Evom
[TM] Epithelial voltohmmeter and an Endohm [TM] tissue resistance measurement chamber, World Precision Instruments, (Sarasota, FL).
The polyolefin phase can bond to polyolefin layers in the structure via diffusion; and the chemical functionality incorporated into the tie resin provides sites for bonding to polar polymers such as nylon and EVOM