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References in periodicals archive ?
Ten intratumoural electrotransfers have been conducted in three European specialised centres.
To this end, B16F10 melanoma tumor bearing mice were intratumorally injected with IL-12 plasmid vector by electrotransfer. The tumor growth and the elicited immune response were evaluated at different posttreatment time points, with a special focus on the macrophage-mediated immune response.
They cover amperometric biosensors, imaging of single biomolecules by scanning tunneling microscopy, applications in reflectivity in bioelectrochemistry, model lipid bilayers at electrode surfaces, enzymatic fuel cells, Raman spectroscopy of biomolecules at electrode surfaces, membrane electroporation in high electric fields, and electroporation for medical use in drug and gene electrotransfer.
In the 2nd dimension, proteins were separated on 12.5% sodium dodecyl sulfate polyacrylamide gel with Protean 11 (Bio-Rad) followed by an electrotransfer to Hybond-P polyvinylidene difluoride membranes (Amersham Biosciences) in Towbin transfer buffer by use of a Protean II Transfer apparatus (Bio-Rad).
After electrotransfer of proteins from SDS-polyacrylamide gels to PVDF membrane, the 17-kDa and 7.4-kDa bands were cut out and placed in the sequencer.
SDSPAGE and electrotransfer to EMD Millipore Immobilon-FL PVDF Transfer Membranes, immunoblotting, detection, and analysis were described previously by Maaty et al.
Samples (10 [micro]g) of protein extract were size fractionated electrophoretically by 10% polyacrylamide SDS-PAGE gel and transferred onto a PVDF membrane using the BioRad Mini Protean electrotransfer system (CA, USA).
Samples (40 [micro]g of protein) were subjected to SDS-PAGE analysis (10-12%) and acrylamide gels by semidry electrotransfer to nitrocellulose membranes (Biorad, USA).
The electrotransfer time was 45 min with a current/area of 0.7 mA/[cm.sup.2].
After electrotransfer at 250 mA for 2 h, each blot was cut into strips with about 3 [micro]g of protein per strip.
After electrotransfer, the membranes were immunostained for TRADD protein by standard Western blot procedure.