The dried microscopic slides were stained with
EtBr (20 [micro]g/mL in distilled water, 60 [micro]L/slide) and were examined with a Leica[R] fluorescence microscope under green light.
To access whether ROS generation leads to the DNA damage in Candida and Mycobacterium, spot assay was performed using
EtBr, a known DNA damaging agent, at a concentration which shows no growth defect in Candida cells.
Parameters Control Chemical mutagens
EtBr EtBr Col.
EtBr with low molecular weight (MW 394 Da) can go through such pore but EthD2 with high molecular weight (MW 1293 Da) cannot.
Synthesized cDNAs were loaded on 1.2% of agarose gel with 10ng/ml concentrated
EtBr ran for electrophoresis set at 100 V.
In the electrophoresis dyes market,
EtBr accounted for the largest share in 2015.
Detection of PCR products: The PCR-amplified products were detected in 1.5% ethidium bromide (
EtBr)-stained agarose gel electrophoresis.
The resultant fragments were electrophoresed on a 2% agarose gel and were visualized by staining the gel with ethidium bromide (
EtBr).
Electrophoresis Reagents Market by Product (Dyes (
EtBr & SYBR) Gels (Polyacrylamide, Starch, & Agarose) Buffer (TBE & TAE)) by Application (Protein Analysis, DNA & RNA Analysis) by Technique (Gel & Capillary Electrophoresis) - Global Forecast to 2020
The resultant DNA was examined on 1.5% agrose-TAE gels, stained with ethidium bromide (
EtBr) and visualized under UV light.
A combination assay between the 13 compounds and the antibiotics erythromycin, tetracycline and oxacillin, and ethidium bromide (
EtBr) was performed to search for antibiotic modulatory activities.
To detect RNA degradation, an aliquot of each total RNA sample was run on an 0.8% agarose gel stained with ethidium bromide (
EtBr).