FAFLP

AcronymDefinition
FAFLPFluorescent Amplified Fragment Length Polymorphism (genotyping technique)
References in periodicals archive ?
Analise dos marcadores fAFLP. Foram selecionados fragmentos com tamanho entre 50 e 500 pares de bases.
Todos os acessos de araca utilizados no presente trabalho e submetidos a analise de fAFLP apresentaram amplificacao para 18 das 24 combinacoes seletivas testadas, conforme exemplificado nas Figuras 1 e 2.
Os marcadores fAFLP selecionados para a analise apresentavam tamanho entre 50 e 500 pares de bases.
Os marcadores moleculares fAFLP tambem permitiram a discriminacao dos genotipos deste estudo em dois grupos (Figura 2), embora as plantas tenham se apresentado com uma distancia genetica inferior quando comparada com a analise morfologica.
All isolates were subjected to FAFLP as described previously (16,17).
FAFLP electropherograms were analyzed using GeneScan 3.7 and Genotyper 3.7 soft wares (PE Biosystems, USA) to detect differentially amplified genomic fragments (amplicons).
FLiP The main advantage is low discrimination power that it is a quick in isolates with less method based on the than six IS6110 copies IS6110 sequence from 1 (22) ng DNA FAFLP Especially useful for Its disadvantages differentiating isolates Include the need of with less than five isolates for culture.
For FAFLP, the selective primer combinations Eco+0 Mse+T, Eco+0 Mse+TA, and Eco+0 Mse+CG were used, and gel separation and fragment analyses were performed, all as described by Scott et al.
De cada planta, foi coletado material para a analise com marcador molecular (fAFLP), visando a comparacao entre a progenie e sua planta-matriz.
1998), it was possible to predict the sizes of DNA fragments generated by fAFLP of that species and demonstrate the high experimental fidelity of the technique (Cousins et al.
Apos a obtencao do material genetico, foi realizada analise visando a deteccao de polimorfismo nos materiais utilizados, por fAFLP, segundo o protocolo AFLP (1997), seguindo as etapas: digestao do DNA; ligacao dos adaptadores; amplificacao pre seletiva; amplificacao seletiva, e eletroforese no sequenciador ABI PRISM 377 DNA Sequencer, utilizando padrao interno de peso molecular Gene Scan-500 ROX, com 15 fragmentos, nos comprimentos em pares de bases (pb): 50; 75; 100; 139; 150; 160; 200; 250; 300; 340; 350; 400; 450; 490 e 500.