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To directly examine the effects of MeSNA on the inhibition of collagenase we used a kinetic assay that examines the breakdown of N(3-[2-furyl]acryloyl)-Leu-Gly-Pro-Ala (FALGPA) to N-(3[2-furyl]acryloyl)-Leu + Gly-Pro-Ala in the presence of collagenase.
The rate of the degradation of FALGPA when exposed to collagenase alone was significantly greater than FALGPA exposed to collagenase + 2.5% MeSNA (rates of -14.99 and -1.49 absorbance units/min, resp.