According to the company, TVB-2640 is an orally bioavailable, first-in-class inhibitor of FASN
, which is a key enzyme in the de novo lipogenesis (DNL) pathway that is responsible for the synthesis of excess fat in the liver of patients with NASH.
Associations of polymorphisms in bovine DGAT1, FABP4, FASN
, and PPARGC1A genes with intramuscular fat content and the fatty acid composition of muscle and subcutaneous fat in Fleckvieh bulls.
Adipocyte RNA was analyzed for relative expression of DIO2, a gene associated with endocrine function (LEPTIN, ADIPOQ), mitochondrial function (cell death activator (CIDEA), ATP synthase (ATP5A), and carnitine palmitoyltransferase 1B (CPT1B)), fatty acid [beta]-oxidation (acetyl CoA dehydrogenase (ACADM)), fatty acid synthesis (acetyl-CoA carboxylase (ACC2), fatty acid synthase (FASN
), and diglyceride acyltransferase (DGAT)), adipocyte function (PPARgamma (PPAR[gamma])), innate immunity (NLRP3), and select proinflammatory or anti-inflammatory cytokines or chemoattractants (tumor necrosis factor alpha (TNF[alpha]), interleukin-1[beta] (IL-1[beta]), and plasminogen activator inhibitor-1 (PAI-1)).
Crews, MD, ScM, FASN
, FACP, Associate Professor of Medicine, Division of Nephrology at Johns Hopkins University School of Medicine.
Recruitment of a purposive sample of Florida school nurses (N = 171) was facilitated by the Florida Association of School Nurses (FASN
), the Florida School Health Association (FSHA), and 12 Florida county school districts.
The membranes were blotted with specific primary antibodies against ACC, [phospho-pACC.sup.Ser79] (Cell Signaling Technology, USA), FASN
(Santa Cruz, USA), and CPT-1a (Abcam, Inc., USA).
Visker (2013) Effect of polymorphisms in the FASN
, OLR1, PPARGC1A, PRL and STAT5A genes on bovine milk-fat composition.
Based on [E.sub.r] and [TPM.sub.ma]: [TPM.sub.s] values, 13 genes (ACSS1, ACSS2, ADFP, CD36, FABP3, FASN
, GPAM, INSIG1, LPL, SCD5, SPTLC1, SREBF1, and XDH; Table 2; Figure 1) were found to exhibit higher mammary expression over skin or muscle; 6 of these 13 genes (ADFP, FASN
, GPAM, LPL, SREBF1, and XDH) further showed preferential expression during adulthood.
In addition, expression of adipogenic genes and adipocyte markers (CEBPa, PPAR[gamma], FABP4, FASN
, leptin, and ADIPQ) was assessed (Supplemental Figure 4).
Polymorphisms of growth hormone (GH) and fatty acid synthase (FASN
) amongst many others may also have an effect.
Real-time PCR results showed that inhibition of mTORC1 activity by rapamycin decreased expressions of lipogenesis enzymes, such as Srebp1c, Acc1, Fasn
, and Scd1 (Figure 3(b)), whereas induced mTORC1 activity by TSC1 knockdown reversely increased expressions of these lipogenesis enzymes (Figure 3(c)).
Overexpression of VEGF-B in the heart also leads to increased fatty acid synthesis via downregulation of phosphorylated 5' AMP-activated protein kinase (pAMPK) and an increase in Malonyl-CoA and fatty acid synthase (FASN