To explore the influence of activation of the sphingosine pathway on cnidarian immune status, aposymbiotic anemones were incubated for 4 h in FASW supplemented with VO or 1 [micro]mol [l.sup.-1] of either SIP, FTY720, or Sph.
Abbreviations: DAF-FM, 4-amino-5-methylamino-2', 7' - difluorofluo-rescein diacetate; DAN, 2,3-diaminonaphtalcne; FASW, filtered artificial seawater; LPS, lipopotysaccharide; MFI, mean fluorescence intensity; NO, nitric oxide; SIP, sphingosine 1-phosphate; Sph, D-sphingosine; SPPase, sphingosine 1-phosphate phosphatase; VO, vehicle only (0.1% v/v dimethyl sulfoxide).