Backfat thickness showed positive correlations with the hepatic mRNA levels of genes for gluconeogenesis, including FBP1 (r = 0.45, p<0.05), LDHB (r = 0.66, p<0.01), PCCA (r = 0.45, p< 0.05), GK (r = 0.53, p<0.05), and GPD1 (r = 0.56, p<0.05).
There are three bypass reactions of gluconeogenesis pathway: conversion of pyruvate to PEP by enzymes PC and PCK, conversion of fructose 1,6-bisphosphate to glucose 6-phosphate by FBP1, and conversion of glucose 6-phosphate to glucose by G6PC.
By correlation analysis, we found significant correlations of expression levels of several genes including FBP1, LDHB, PCCA, GK, and GPD1 with backfat thickness.
PC, pyruvate carboxylase; PCK2, mitochondrial phosphoenolpyruvate carboxykinase; PCK1, cytosolic phosphoenolpyruvate carboxykinase; FBP1, fructose 1,6-bisphosphatase; G6PC, glucose 6-phosphatase.
Our initial mass spectrometric analysis of proteins fractionated from menin immunoprecipitates identified FBP1 as a menin-interacting partner.
FBP1 plasmid was obtained from OriGene (Rockville, MD).
FBP1 C-20 (catalog number sc-11101; 1: 200 dilution), MYC 9E10 (catalog number sc-40; 1: 200), and HA Y-11 (catalog number sc-805; 1: 1000) antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA).
Menin Binds to FBP1. To identify menin interacting proteins, we conducted a large-scale menin immunoprecipitation using HeLa nuclear extracts and either the SQVb antibody (against C-terminal menin) or H-MEN1b antibody (against full-length menin).
Human fructose-1,6-bisphosphatase gene (FBP1): exon-intron organization, localization to chromosome bands 9q22.2-q22.3, and mutation screening in subjects with fructose-1,6-bisphosphatase deficiency.
A PCR-RFLP approach was applied to detect polymorphisms in the bovine FBP1. The PCR products for FBP1 were digested with Msp I at 37[degrees]C for 4 hrs.
A 7000-rad whole-genome cattle-hamster RH panel (SUNbRH) consisting of 92 hybrids was used to map the FBP1 gene with the primer pair F1-R (Table 1).
A BLAST search against the bovine EST database (http://www.ncbi.nlm.nih.gov/blast/) with the human FBP1 mRNA (NM_000507) resulted in eight ESTs for the bovine FBP1 gene.