Based on the surveillance case definition (2), FDOH
determined that sulfuryl fluoride exposure was the most likely cause of illness among these five family members.
Following manufacturers' instructions, all genomic DNA extractions were either performed using the Epicentre (Qiagen Inc, Madison, WI) extraction kits, a MagNaPure Compact automated instrument (Roche, Inc, Indianapolis, IN, USA), or a boil preparation method used by FDOH
. The boil preparation, (adapted from a reference lab in the Laboratory Response Network) is described as follows: bacterial growth from an overnight cultured media plate was removed and placed into 100 [micro]L of sterile water and boiled for 5 minutes and then placed onto ice for 2 minutes, followed by centrifugation at 12,000 xg for 10 minutes at 4[degrees]C.
During 2009-2010, serum samples from patients with suspected dengue were received by the FDOH
for dengue diagnostic testing; the samples came from 16 of Florida's 67 counties.
The CHDs communicate updates to the public, policy makers, internal/ external response partners, and the media through coordination with the state health office (FDOH
Following manufacturers' instructions, all genomic DNA extractions were performed using the Epicentre (Qiagen Inc., Madison, WI) extraction kits, a MagNaPure Compact automated instrument (Roche, Inc., Indianapolis, IN, USA), or a boil preparation method used by the Tampa FDOH
, a reference lab in the Laboratory Response Network, and is described as follows.
Because the clinical features of dengue are often nonspecific, transmission was likely ongoing before the index case-patient's condition was diagnosed and FDOH
Jackow, Florida Department of Health (FDOH
), for specimen collection.
Because accreditation requires applicants to have a CHIP, the FDOH
felt strongly that EnvPHPS and PACE EH particularly bolstered PHAB Domain 4 ("Engage with the community to identify and address health problems") and should be part of community health improvement planning.
Initial testing at FDOH
laboratories of a serum specimen collected April 13 detected IgM antibodies against both dengue and West Nile virus.
We initiated enhanced surveillance locally through alerts to medical examiners and statewide through requests to laboratory directors to forward to the FDOH
laboratories any cultures suspicious for Bacillus species isolated from sterile sites.
Following the occurrence of three fatal PAM infections in Florida in 2007, FDOH
established a Florida Naegleria work group and assisted in establishing a national Naegleria work group sponsored by the Council of State and Territorial Epidemiologists (CSTE) and CDC (CSTE, 2011).
Aggregated BMI data, by grade and county, are reported annually to the Florida Department of Health (FDOH