A recording electrode was inserted into the CA1 region of the hippocampus (1.5 mm posterior to bregma and 1 mm lateral to the midline) to record field excitatory postsynaptic potentials (fEPSPs
) in stratum radiatum.
Perfusion of asiatic acid had no significant effect on fEPSP
amplitudes, either in slices with concentration asiatic acid 30 [micro]M (Fig.
Therefore, in the present study we tested whether TDE can modulate dendritic field excitatory postsynaptic potentials (fEPSPs
), which indicate the neuronal synaptic function, in Cornu Ammonis 1 (CA1) hippocampal slices of normal rats as found in current AChE-I drugs used for AD therapy.
1A, solid bar; 7.0 [+ or -] 3.8%, n = 3)--the percent change from baseline of the fEPSP
slope, measured at 60 min post-TBS, was substantially reduced when compared with artificial cerebral spinal fluid (aCSF) controls (Fig.
Figure 1 shows averaged raw data traces of the fEPSP
before and after tetanic stimulation.
This was shown by impaired stimulus-response relationship for the slope of the field excitatory postsynaptic potential (fEPSP
) in NL1 knockout (KO) mice.
Regarding extracellular recording, a glass pipette filled with 3 M NaCl was placed in the stratum radiatum of the CAl area to record field excitatory postsynaptic potentials (fEPSPs
Hussain and colleagues found that, while in vivo PCB exposure does not produce any obvious baseline changes in synaptic response prior to stimulation, it reduces the increase in fEPSP
after stimulation in a dose-dependent fashion.
(a) Normalized fEPSP
slopes of HFS-induced LTP and (b) TBS-induced LTP.
In sham controls, fEPSP
slopes were 110 [+ or -] 4.2% (n = 6) of baseline after LFS, an increase that was not statistically significant (p = 006) (Figure 3(b)).
A single 100 Hz tetanus (1 s) is applied (stim), to induce LTP (recordings of field excitatory postsynaptic potentials; fEPSP
) in slices prepared from either pilocarpine- or vehicle-injected animals (stratum radiatum, rad; stratum lacunosummoleculare, la.-mol.; stratum moleculare, mL; dentate gyrus, DG; nuclear stain, TO-PRO; scale bar: 200 [micro]m).
To examine the effect of TPPU on basal excitatory synaptic transmission, we measured the synaptic response by recording the fEPSP
in area CA1 of the hippocampus.