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In all treatments, MMP-2 was expressed the most at 18 h, and the highest expression was found in FFBO compared to other treatments.
The expression of TIMP-2 in spermatozoa protein was similar to that in the culture medium, and it was expressed more in FBO and FFBO than in LFBO and LuFBO.
In FBO and FFBO, the expression was distinguished in the head of the spermatozoa, while it was not detected at all in LFBO and LuFBO.
The TIMP-3 expression pattern was similar to that in culture media: TIMP-3 expression was higher in FBO and FFBO. In LFBO, the TIMP-3 expression rate was higher than that in other culture media.
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