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Several neurotrophic factors are released and act together after peripheral nerve injury to stimulate neural regeneration, including nerve growth factor (NGF), a brain-derived neurotrophic factor (Boyd & Gordon), neurotrophin 3 (NT-3) (Markus et al., 2002), insulin growth factors (IGFI and IGF-II) (Perlson et al., 2004), and fibroblast growth factor 2 (FGF-2) (Terenghi, 1999).
To determine whether aerosol GCB affected tissue remodeling (the third phase of wound healing), we quantified IL-10 and FGF-2 (bFGF), both of which are critical cytokines for the remodeling phase of wound healing.
Welter, "FGF-2 enhances the mitotic and chondrogenic potentials of human adult bone marrow-derived mesenchymal stem cells," Journal of Cellular Physiology, vol.
It has been observed that among basal proliferative, angiogenetic, and chemo-attractive proteins, such as VEGF, PDGF, HGF, and ANG-2, MenSCs secrete biologically active molecules IGF-1 and FGF-2, which are involved in different stages of chondrogenesis (Figure 3) [25, 53], as described earlier.
Chatterjee, "Neuron-microglia crosstalk up-regulates neuronal FGF-2 expression which mediates neuroprotection against excitotoxicity via JNK1/2," Journal of Neurochemistry, vol.
For FGF-2 staining, sections were submerged into a 0.3% Triton X-100 solution for 15 minutes and rinsed three times for 5 minutes with phosphate-buffered saline (PBS).
FGF-2 protects ischemic tissue by activating the anti-apoptotic protein (22).
PDGF and FGF-2 expression were measured by Western blot.
FGF-2, VEGF, and PAI-1 were measured by an immunological-based fluorescent multiplex assays (Human CDV1, Millipore, Billerica, MA, USA) using a Bio-Plex 200 system (BioRad), which utilizes Luminex xMAPTM fluorescent bead-based technology (Luminex Corp., Austin, TX).
In this study, time-course changes in HIF-1[alpha] and HGF, IGF-1, and FGF-2 mRNA expression were similar.
Thus, the present study aimed to evaluate the morphological changes, growth, and plasticity of mesenchymal cells of a culture medium conditioned by explants of the facial nerve and the presence of FGF-2.
Quantification of growth factors in PRP and platelet-poor plasma (PPP) fractions revealed increased levels of mitogenic growth factors PDGF-AB, PDGF-BB, and FGF-2, the angiogenic agent VEGF and the chemokine RANTES in ucPRP compared to adult PRP (aPRP) and PPP.
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- FGF receptor
- FGF-inducible kinase