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In multiplex assay, the levels of eight biomarkers (including CSF-2, IL-5, IL-9, IL-12p70, IL-13, IL-17, FGFb, and TNF) were below the limits of detection in all patients in both baseline and SVR times.
This pattern was statistically significant in anti-inflammatory cytokines, IL-4 (P = 0.034), IL-10 (P = 0.021), and TGF-0 (P < 0.0001) and proinflammatory cytokines, IFN-[gamma] (P = 0.008), IL-10 (P = 0.027), and IL-15 (P = 0.026), as the same as for other molecules, FGFb (P <0.0001) and PAI-1 (P = 0.002).
Serum levels of growth factors revealed a decreasing pattern associated with an increase in liver disease severity; however, significant findings were only observed in FGFb (P = 0.019).
The mouse primary antibodies to FGFb were diluted 1: 100.
Analysis of Granuloma Cells for Production of IFN[gamma], IL-1[alpha], GM-CSF, and FGFb. It is well established that cytokines IFN[gamma], IL-1, and GM-CSF are inflammatory mediators that activate and differentiate immune cells increasing their microbicidal potential in response to infection by intracellular pathogen thus playing an important role in initiating, regulating, and control of inflammation [3-5, 8, 12].
It is probably the granuloma cells producing GMCSF and FGFb growth factors that promoted their survival in both compact structures of granulomas and ex vivo culture.
(3) Basic medium, OIM, and rhFGF-basic (FGFb) (R&D, cat.
No folding was seen in the FGFb 10 ng/ml group, and a better visualized ARS was observed after 14 days.
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- FGFR signalling adaptor
- FGFR substrate 2
- FGFR-signalling adaptor SNT