To identify the role of HIF1[alpha], we first examined the FGFRL1 expression level after HIF1[alpha] silencing with siRNAs under hypoxia condition for 6 hours.
FGFRL1 Affects OC Cell Proliferation, Apoptosis, and Cell Migration In Vitro.
FGFRL1 Regulates the Hedgehog (Hh) Signaling Pathway.
To further evaluate whether knockdown of FGFRL1 inhibited downstream Hh signaling, the Gli-luciferase reporter was used in luciferase reporter assays.
In this study, we observed that FGFRL1 was commonly upregulated in both OC cells and tissues compared with normal controls.
FGFRL1 was demonstrated to express preferentially in skeletal tissues, and small amounts of FGFRL1 mRNA were detected in other tissues such as the heart .
To evaluate the prognostic value of FGFRL1 in OC, we performed Kaplan-Meier survival analysis.
It was reported that downregulation of FGFRL1 decreased cell proliferation by promoting the proportion of cells in G1/G0 phase and decreasing in S and G2/M phases in human laryngocarcinoma cancer and esophageal squamous cell carcinoma [14, 16].
FGFRL1 was ever thought to have no effect on ERK1/2 signaling without the intracellular tyrosine kinase domain .
In conclusion, we describe FGFRL1 as a crucial factor in the clinical outcome and progression during human OC, indicating it is a novel therapeutic target that can be used for the treatment of OC.
Our results demonstrated that FGFRL1 was commonly upregulated in OC cells and tissues compared with normal controls.
Supplementary Table S3: correlations between FGFRL1 expression and clinicopathologic features in OC patients.