The codon-optimized FGH (oFGH) sequence was synthesised according to the P.
Purification and administration methods of recombinant fish growth hormone (FGH) are the main concerns of impracticable use in mass-scale aquaculture.
Further analysis by SDS-PAGE under denaturing and nondenaturing conditions showed that both FGH forms (nFGH and oFGH) were produced by P pastoris as monomers and multimers (Figure 3).
The main outcome of the high content of oFGH in the culture supernatant is enhancing the bioavailability of FGH that essentially stimulates fish appetite as well as feed conversion rate [21, 22].