The results of MMP levels are presented as the FITC-positive cells/total cells (corresponding
FITC geometric mean).
In the first tube, cells were labelled using phycoerythrin (PE) conjugated monoclonal antibody (MoAb)for surface markers CD3 (BD Biosciences Immunocytometry Systems, San Jose, CA) and fluorescein isothiocyanate (
FITC) conjugated antibody for CD25 (BD Biosciences Immunocytometry Systems, San Jose, CA).
Light green color florescence emitted by the intraerythrocytic parasites under dark green background, observed with fluorescein isothiocyanate (
FITC) filter in a florescent microscope (Fig.
Hidden from view, the
fitC takes up no space and you'll soon forget it's there.
The fluorescent probes FITC-iCREKA and FITC-CREKA were prepared using
FITC, a derivative of fluorescein.
To increase the detection efficacy,
FITC was attached to UDCA.
The stained samples were examined at excitation/emission wavelengths of 405/461 nm for DAPI and 490/530 nm for
FITC.
Por lo tanto, el objetivo del presente trabajo fue determinar el porcentaje de integridad acrosomal en espermatozoides epididimarios de alpaca, utilizando Arachis hypogaea (PNA) y Pisum sativum (PSA), conjugadas con
FITC y evaluadas mediante citometria de flujo con sistema analizador de imagenes.
CD: cluster of differentiation, APC: allophycocyanin, PE: Phycoerythrin,
FITC: fluorescein isothiocyanate, and PE-Cy7: R-Phycoerythrin-Cyanine 7.
MitoTracker, DAPI, and
FITC dyes were excited with 561,405, and 488 nm laser, respectively, at 2 mV.
For example, anchored VWF strings have been visualized after perfusion of fluorescently labeled platelets [26] or using an FITC-conjugated polyclonal anti-VWF antibody (VWF: IgG:
FITC) [25].
The evaluation of immune cells status in blood was measured through full blood count, standard panel of T-cells (CD3), B-cells (Mouse anti Rabbit IgM), and subset T-Lymphocyte (Mouse anti Rabbit CD4, Mouse anti Rabbit CD8) using Flow Cytometry (BD FacsCanto II) Technique, included mixture kits of antibody CD45 APC, antibody CD14 PE, antibody T-Lymphocyte
FITC, antibody B-cells
FITC, antibody CD4
FITC, and antibody CD8
FITC), using 4 ml of whole blood sample (EDTA tube).
