CH (75% deactivated, molecular weight 195 kDa), HA, FITC
, and BSA were purchased from sigma Aldrich Bangalore, India.
The significance of the effects of FITC
conjugation, heating temperature, and their interaction was analyzed by a two-way analysis of variance (ANOVA).
4] Nonstandard abbreviations: PTCA, percutaneous transluminal coronary angioplasty; ASA, acetylsalicylic and (aspirin); FACS, fluorescence-activated cell sorting; PKA, cAMP-dependent protein kinase; P-VASP, phosphorylated vasodilator-stimulated phosphoprotein; EIA, enzyme immunoassay; ABTS, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt; FITC
, tluorescein isothiocyanate; PRP, platelet-rich plasma; PBS, phosphate-buffered saline; MFI, geometric mean fluorescence intensity; PRI, platelet reactivity index; and [PGE.
4] Nonstandard abbreviations: TIL, tumor-infiltrating lymphocyte; TCR, T-cell receptor for antigen; SEB, staphylococcal enterotoxin B; PBMC, peripheral blood mononuclear cell; FITC
, fluorescein isothiocyanate; FACS, fluorescence-activated cell sorting; PE, phycoerythrin; PerCp, peridinin chlorophyll-[alpha] protein; and SSC, standard saline citrate.
1] Nonstandard abbreviations: HCV, hepatitis C virus; UTR, untranslated region; RT-PCR, reverse transcription-PCR; FRET, fluorescence resonance energy transfer; FITC
, fluorescein isothiocyanate; and [T.
4] Nonstandard abbreviations: PV, platelet volume; PC, platelet component; MPC, mean PC; MPV, mean PV; ACD, acid-citrate-dextrose; CTAD, citrate, theophylline, adenosine, and dipyridamole; E/C, tripotassium EDTA plus CTAD; TS; Tyrode's salt solution; FITC
, fluorescein isothiocyanate; and PE, phycoerythrin.
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H], immunoglobulin heavy chain constant domain; MPBS, milk powder phosphate buffered saline; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; Ap, alkaline phosphatase; and FITC
, fluoresceine isothiocyanate.
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/CD14-PE, isotype control, CD3-FITC
/CD 19-PE, CD3-FITC
/CD16-PE and/or CD3-FITC
/CD56-PE (PE, phycoerythrine; FITC
, fluorescein isothiocyanate)].
The probes are available with three different labels, Cy3, FITC
Stained cells were counted after visualization by a fluorescence microscope equipped with a FITC