The cycling conditions used were as follows: initial denaturation of 94 [degrees]C for 5 minutes; followed by 35 cycles of 95 [degrees]C for 50 s; 61 [degrees]C for 50 s (cctA), 62 [degrees]C for 50 s (nanA) or 68 [degrees]C for 50 s (fliC
); and 72 [degrees]C for 7 minutes.
coli motility, whereas QS-II expression enhances motility by affecting fliC
Our review of the literature reveals that some of the best work on FLIC
comes from graduate students.
operates a factory in central Taiwan with annual capacity of 4,800 tons.
The amplified fliC
gene was cleaved with HhaI restriction endonuclease (Invitrogen), when fliC(M) primers were used, and RsaI restriction endonuclease (Invitrogen), when fliC(F) primers were used.
Northwest-based relationship writer Flic
Everett says: ``It's all very well fancying the person you're with but if the environment isn't right, there is less chance of creating that perfect romantic mood.
Three pics funded by the FLIC
scheme have been released.
Sex expert Flic
Everett analysed the responses, taken from five UK cities, and concluded dance floor duffers are unlikely to score highly in the passion stakes.
These results suggested that the 38 kDa is the major structural component (FliC
) of the flagella filament.
Johns captures perfectly, if melodramatically, the fears that had both driven and sustained Johns's brainchild, the Florida Legislative Investigation Committee (FLIC
)-popularly known as the Johns Committee--for the previous five years.
EVERETT: "It's okay to sleep with him if you want to, but it helps if you wait at least a few days - and make sure you're sober when you do it.