In the present study, the dataset from Huashan Hospital was analyzed using FLICA, which was run totally on MATLAB R2014a installed in a Linux environment.
The independent components (ICs) output from FLICA contained three types of information: IC values, spatial information, and voxel values (see Table 2).
Exploration of the Initial Age-Related Brain Regions Using FLICA. A total of 29 ICs were obtained from the FLICA blind tests for any other demographics or cognitive factors of the participants.
The automatic results from FLICA showed that IC3 totally contained 128271 voxels, with voxel values ranging from 1 to 38.
Therefore, the FLICA method is very suitable for the present study.
Quantification revealed significant increases in FLICA+ (b), TUNEL+ (c), and Annexin V+/propidium iodide- (PI-) cells (d) by 8h (FLICA and Annexin V) and 16h (TUNEL) after cytomix.
(a) Cytomix stimulation leads to an increased number of cells stained positive for both active caspases (FLICA; red) and fragmented DNA (TUNEL; green).
Annexin V; FLICA TEER, Yes 2017 (present (pan-caspase EB-albumin study) activity); TUNEL Citation Comments Bannerman et Similar time course of leak and apoptosis; al.
The correlation (Pearson test) between fluorescence intensity of the FLICA labeling (present results) and the regenerative ability of the descending neurons  was analyzed.
Also, previous studies revealed that bad regenerator/poor survivor neurons show high levels of activated caspases in the first 2 weeks following a complete SC transection as revealed by FLICA labeling [30-32] and that intense FLICA labeling and TUNEL staining are observed in bad regenerators several weeks after a complete SC transection .
In previous studies, we reported intense FLICA labeling 2 weeks after lesion in bad regenerators using the FLICA reagent specific for the detection of activated caspase-8 (FAM-LETD-FMK) .
Incubation of the brains with the FLICA reagent FAMDEVD-FMK revealed the presence of activated caspase-3 or caspase-7 in identified SC projecting brainstem neurons at 10 weeks posttransection (Figure 3), but not in neurons of untransected control animals (Figure 3(a)).