:EGFP)y1 transgenic embryos were incubated with 1,3, and 10 [micro]g/mL of DOE from 24 to 72 hours after fertilization (hpf)).
Among the flagella class-II structural proteins, ORFs for L-ring protein FlgH (RWLH05339), P-ring protein FlgI (RWLH04317, RWLH05340), flagellum-specific muramidase FlgJ (RWLH04318, RWLH05341), flagellar protein FliO (RWLH00722), flagellar basal body-associated protein FliL
(RWLH00719), flagellar FliJ protein (RWLH00717), flagellum-specific ATP synthase (RWLH00716, RWLH04297) and assembly protein Flk (RWLH01371) were identified.
Using biolayer interferometry (BLI) and surface plasmon resonance (SPR), we have undertaken a survey of protein-protein interactions among the soluble apparatus proteins (FlhAc, FlhBc, FliH, Flil
, FliJ, FliK) and between apparatus proteins and export substrates.